المصدر
Journal of Agricultural and Marine Sciences (JAMS), v. 29, no. 2, p. 33-41.
الملخص الإنجليزي
Epithelial ovarian cancer (EOC) is a common gynecological cancer and a leading cause of death, es
pecially because the tumors develop resistance to cisplatin. New compounds are needed to achieve better disease
control and survival. We examined the cytotoxic effect of Gallic acid (GA), Hymenialdisine, and Malformin A1
(MA1) on human ovarian cancer cells. Cytotoxicity was tested using cisplatin-sensitive (A2780s) and cisplatin-resis
tant (A2780cp) ovarian cancer cell lines, and a normal ovarian tissue cell line (HOSE6-3) using AlamarBlue assay,
Hoechst dye, and flow cytometry, and the genes and proteins of interest were assessed using western blot, and qRT
PCR. The IC50
of Hymenialdisine was 146.8 μM for A2780s cells and >300 μM for A2780cp cell lines. Both GA and
MA1 decreased cell viability in a concentration-dependent manner. The IC50
of GA was 103 µM for A2780s cells, 189
µM for A2780cp cells and 262 µM for HOSE6-3 cell lines, for MA1 IC50
was 0.23 µM for A2780s and 0.34 µM for
A2780cp. This was in comparison to IC50
of 31.4 µM and 76.9 µM, for A2780s cells, and A2780cp cells respectively
for cisplatin. The combination of GA and MA1 with cisplatin revealed synergistic action, especially in A2780cp cell
lines. The results suggest that both GA and MA1 may help overcome the resistance to cisplatin through the synergistic
effect. Hence, the cytotoxic potential of GA and MA1 merit further investigation.
