الملخص الإنجليزي
Objectives: We sought to determine the prevalence of asymptomatic bacteriuria (ASB)
in patients with sickle cell disease (SCD), the susceptibility profile of its agents and
their extended-spectrum β-lactamase (ESBL) production. Methods: Fifty-nine
patients with SCD and 116 healthy controls were investigated. Urine samples were
collected and cultured by standard techniques. We used the disc diffusion technique to
determine antibiotic susceptibility. ESBL was detected by the combination disc method
and detection of blaSHV, blaTEM, and blaCTX-M genes by multiplex-polymerase chain
reaction. Results: The prevalence of ASB was higher among patients with SCD (8.6%)
than controls (0.9%) (p = 0.016), predominantly among females. Coagulase-negative
staphylococci (n = 2; 33.3%) predominated among the isolates. Other uropathogens
included Stenotrophomonas maltophilia, Acinetobacter baumannii, and Enterobacter
cloacae. All isolates were sensitive to meropenem but were resistant to ceftazidime,
ampicillin, and tetracycline. blaSHV, blaTEM, and blaCTX-M-15 were detected in Enterobacter
cloacae. Conclusions: The prevalence of ASB is high in patients with SCD predominantly
among females. Rare multidrug-resistant uropathogens were implicated. We posit a need
for resistance surveillance programs and antibiotic stewardship to prevent treatment
failure and reduce drug resistance.
