الملخص الإنجليزي
Vitamin D3 deficiency is being evaluated at Sant Parmanand Hospital, a 140-bed, tertiary care,
multidisciplinary private hospital in Delhi, India. With effect from April 2010, 25-hydroxy vitamin D
[25(OH)D] levels are measured in the hospital laboratory using commercially available ELISA kits. We use
the 25(OH)D direct Elisa Kit (Immundiagnostik AG, Bensheim, Germany), based on a competitive ELISA
technique with a selected monoclonal antibody that recognises 25(OH)D. The results are expressed, after
point-to-point calculation, as nmol/l (with 1 nmol/L being equivalent to 2.5 ng/mL). Values ≥80 nmol/L
correspond to a sufficient level of vitamin D, while those of <50 nmol/L to a deficient, and 50–75 nmol/L to
an insufficient level.
