الملخص الإنجليزي
Objectives: This study aimed to document the association of human papilloma virus (HPV) and
its types in breast carcinoma tissues in Kuwaiti women, and correlate this with known prognostic markers.
Methods: The clinicopathological data of archived tissue from 144 cases of invasive ductal breast carcinoma were
studied (age, histological grade, size of tumour, lymph node metastases, oestrogen/progesterone receptors and
human epidermal growth factor receptor 2 status). HPV frequency was documented using immunohistochemistry
(IHC) and chromogenic in-situ hybridisation (CISH). HPV types were documented by CISH using HPV probes.
CISH and IHC techniques were compared and HPV correlated with prognostic parameters. Results: The HPV
prevalence as determined by CISH and IHC was 51 (35.4%) and 24 (16.7%) cases, respectively. The sensitivity of
HPV by IHC was 37.3% and specificity was 94.6%. The sensitivity and specificity of HPV-CISH compared to HPV IHC was statistically significant (P <0.001). HPV-CISH was seen in 51 cases. A combination of HPV 6 and 11, and
16 and 18 was seen in 2 (3.9%) cases, and a combination of HPV 6, 11, 31 and 33 was seen in 7 (13.7%) cases. All
three HPV probes: 6 and 11, 16 and 18, as well as 31 and 33 were present in 2 (3.9%) cases. The prevalence of HPV CISH in the Kuwaiti and non-Kuwaiti populations was 27 (52.9%) and 19 (37.2%), respectively. No correlation was
observed with the prognostic parameters. Conclusion: The frequency of HPV in breast carcinoma cases in Kuwait
was 35.4% (CISH). Of those, 52.9% were Kuwaitis in whom both low- and high-risk HPV types were detected.
