Gentic diversity and variation in resistance to hymexazol among and within fusarium solani populations obtained from citrus, cucumber and date palm in Oman
مؤلف
Al-Masoudiyah, .Ruqaya Salim Mohammed
الملخص الإنجليزي
A study was conducted to investigate diversity among 80 Fusarium solani isolates obtained from date palm (30), citrus (31) and cucumber (19). Characterization based on sequences of the EFla and ITS rDNA genes of the 80 Fusarium solani isolates showed grouping of the isolates in two major clusters, with no relationship with the host or geographical origin from where the isolates were obtained. AFLP analysis using 3 primer pair combinations produced 980 polymorphic and 80 AFLP genotypes. Populations from different hosts and districts showed moderate levels of genetic diversity (H= 0.2494). Clustering of the isolates was not related to host or geographical origin of the isolates. This was supported by analysis of molecular variance (AMOVA) which Un indicated the existence of low level of genetic differentiation among populations obtained from different hosts (Fst = 0.0162) and regions (Fst=0.0066). F. solani isolates displayed variation in sensitivity to hymexazol, with ECso values ranging from 2 to 5745ug mil (meán = 878 ug ml,'). This implies the existence of resistance to hymexazol among F. solani isolates in Oman. The study provides evidence for frequent movement of F. solani inoculum among hosts and regions in Oman, which could be attributed to cultural practices employed by farmers. Growers should move towards the use of integrated disease management strategies to avoid further development mers. em of resistance to hymexazol.
