الملخص الإنجليزي
A new, simple, sensitive and rapid reversed-phase high performance liquid chromatography (RP-HPLC) method is proposed for the analysis of environmentally important phenols in water. The use of coumarin-6-sulphonyl chloride (C6SCI) as a fluorescence labeling reagent has been investigated. The compound reacts with phenols within 20 minutes under mild conditions (ambient temperature) to give sulphonates that can be separated by RP-HPLC employing fluorescence detection at Rex=360 and hem=460 nm. The optimum conditions for fluorescence, derivatization and chromatographic separation have been established and detection limits in the range 0.1-0.9 ug 1 were obtained for the studied were compounds. Extraction and preconcentration steps were not necessary. The calibration curves were linear for the range 6-200 ug l'for phenol, 3-200 ug r' for2-chlorophenol, 4-chlorophenol and 2,3,5-trichlorophenol and for the range of 3 100 ug r' for 2,3-dichlorophenol and 3,5-dichlorophenol. The practical applicability of the method to environmental samples was demonstrated by analyzing drinking and industrial water samples spiked with the phenolic compounds.
