الملخص الإنجليزي
Gastric cancer pathogenesis is caused by nitrosamines compounds that are introduced to the human food chain as in specific food products, including processed fish products by salting or drying. In Oman there is no studies conducted to investigate the association between gastric cancer and traditional fish products (dried and salted fish). Objective: This study aimed to investigate the protective effect of curcumin against the gastric cancer pathogenesis-associated with dried and salted fish feeding as compared to a rat model of gastric cancer induced by nitrosamine injection. Methods: This in vivo experiment included nighty six male Sprague Dawely male rats (12 groups, n=8/group): Negative control group, nitrosamines-injected group, curcumin supplemented group, and nitrosamines injection plus curcumin supplementation. The other eight groups were fed an experimental diet that contains either dried fish (Anchovies and Shark) or salted fish (Yellow Fin Fish and Queen Fish) in the presence or absence of curcumin supplementation. The rats were exposed for this feeding protocol for eight weeks and then sacrificed. The gastric tissue was dissected and divided into two parts: the first part was homogenized and used for biochemical measurements of total antioxidant capacity (TAC), glutathione (GSH), and antioxidant enzymes (Catalase, Glutathione peroxidase, Glutathione-S-transferase, and Superoxide dismutase). Meanwhile, the second part was examined for histopathological changes. Results: Dried and salted fish feeding revealed a similar action as nitrosamines injection and caused oxidative stress and abnormal histological morphology in the examined gastric tissues as evidenced by impairment of TAC, GSH depletion and inhibition of antioxidant enzymes. Curcumin supplementation has significantly abrogated the observed oxidative stress-associated with dried and salted fish feeding. Conclusion: Curcumin supplementation combats oxidative stress and its associated gastric cancer pathogenesis in the experimental animals treated with nitrosamine or fed on a diet rich in dried and salted fish products.
