English abstract
Objectives: In preterm infants, hyperbilirubinemia is common and can impair the
central nervous system. The tests available for measuring bilirubin is to collect blood
from heel pricking and occasionally taking blood samples from inserted cannulas, which
is painful. Therefore, there is a need to develop a non-invasive device to detect bilirubin
levels in newborns and interpret the severity of jaundice. Methods: We conducted a
cross-sectional study of 100 neonates. Patient data was collected between June 2015 and
December 2016 from King Khalid Hospital at Al-Majma'ah, Saudi Arabia, and Alpine
Hospital, Gurgaon, India. The mean gestational age of neonates was 39.0 weeks. Total
bilirubin was measured using a transcutaneous bilirubinometer on the forehead and
obtaining optical imaging through scanning of conjunctiva of eyes, also referred to as
BiliChek and BiliCapture, respectively. Later the blood samples were obtained from
these patients and tested in the laboratory to determine total serum bilirubin (TSB)
levels. Results: The concentration of bilirubin as measured from serum, BiliChek, and
BiliCapture were 10.7±2.0, 11.6±2.7, and 13.1±2.3 mg/dL, respectively. Correlation
was high between TSB and BiliChek (r2
= 0.88) and between TSB and BiliCapture
(r2 = 0.73). The Bland-Altman plots showed good agreement when comparing bilirubin
values for both BiliChek and BiliCapture devices. Bilirubin measurement was further
checked for the sensitivity and specificity and was 88.0% and 76.0% using BiliChek and
92.0% and 75.6% using BiliCapture, respectively. Conclusions: The optical imaging of
conjunctiva for bilirubin assay is a safe alternative to a laboratory bilirubin assay and
transcutaneous bilirubinometer BiliChek.
