English abstract
Pompe disease (glycogen storage disease type II) is a rare autosomal recessive lysosomal
storage disease that is caused by acid alpha-glucosidase deficiency. Early enzyme replacement therapy can benefit
infants with the disease but the diagnosis is complicated by the rarity of the disease and the heterogeneity of the
clinical manifestations. In this study, DNA extracted from archival postmortem formalin-fixed paraffin-embedded
tissues was used to identify Pompe disease mutations in Oman and develop a rapid molecular-based test. Methods:
Intronic primers were designed to amplify short fragments (193–454 base pairs [bp]) from coding exons (2–20) and
screen for mutations using direct sequencing (DS). Results: Two mutations known to cause severe disease were
identified in two samples. One was a coding mutation, c.2560C>T (p.Arg854X), and the second was found at a splice
acceptor site, c.1327-2A>G. Polymerase chain reaction- and restriction fragment length polymorphism-based tests
were designed for the rapid genotyping of the identified mutations. Conclusion: These tests can facilitate prenatal
diagnosis and help in identifying carriers in families with the identified mutations.
