English abstract
A simple, rapid and sensitive method has been developed for analysis of mebeverine (MEB) in pharmaceutical formulations using tris (2,2'-bipyridyI) ruthenium(II) chloride (Ru (bpy)325) peroxydisulphate chemiluminescence (CL) system in a Two-chip system. Various parameters that influence the CL signal intensity were optimized. These include pH, flow rates, volume and concentration of reagents used. Under optimum conditions, a linear calibration curve between (0.2-4) ug mL-' was obtained (regression liner >0.9914). The detection limit was 0.043 ug mL-', while the limit of quantification was found to be 0.138 ug ml-!. The developed method is fast, cost effective and environmentally friendly method. Moreover, a new mechanism for (Ru(bpy)321-perxodisulphate (S2082) chemiluminescence (CL) system has also been proposed. The mechanism is based on photoinduced oxidation of Ru(bpy)32+ to Ru(bpy)33*via formation of Ru(bpy)32+* upon irradiation with visible light. S2002- then oxidizes Rusbpy);2+* to Ru(bpy)33+ and subsequently the analyte reduces the resultant Ru(bpy)33+ to Ru(bpy)32** which then produces the CL signal. The procedure was applied to the analysis of MEB in pharmaceutical products and biological samples and was found to be free from interferences from concomitants usually present in these preparations. The number of samples which can be analysed in an hour by this method reach 180 sample with the lowest consumption of chemicals about 17uL per run.
