English abstract
Two highly sensitive and selective luminescence methods for the assay of
Amlodipine (AM) and Lisinopril (LN) in pharmaceutical formulation and biological
fluids were described.The method is based on the luminescence sensitization of terbium (Tb3) by complexation with AM. The signal for AM -Tb was monitored at Nex=242 nm and
Tem=548 nm . Optimum condition for the formation of the complex in aqueous media
were 60 uM of Tb34, 0.16% micellar solution of 4-dodecylbenzene sulfonate acid
(SDBS), 0.015M of Tris buffer buffered at pH=9, 0.1 mM of tri-n-octylphosphine
oxide (TOPO) as a synergic agent and the best co-luminescence was Eu34 with
concentration 2x10,M where 0-100 ppb of AM was determined in the batch method
with LOD= 1.2 ppb. The same method was applied for the assay of LN. The method is based on the luminescence sensitization of terbium (Tb37) by complexation with LN. The signal
for LN-Tb was monitored at hex=240 nm and hem=548 nm . Optimum conditions for
the formation of the complex in aqueous media were 80 uM of Tb", 0.1% of
(SDBS), 0.01M of Tris buffer buffered at pH=10, 60 uM of (TOPO) as a synergic
agent and Eut with concentration 1 uM as columinescence where 0-1000 ppb of LN
was determined in the batch method with LOD= 19.9 ppb.
