Composition and anti-bacterial properties of steam volatiles from cleome austroiranica, blepharispermum hirtum and physorhynchus chamaerapistrum
Author
Al-Mughairiyah, Baqiya Ali Khalifa
English abstract
This thesis reports the composition and biocidal properties of steam-distilled volatile oils from Cleome austroiranica Delile., Blepharispermum hirtum Oliv. and Physorhynchus chamaerapistrum Boiss. The components of the oil were determined by GC/MS and 'SC NMR analysis. The two major components of C. austroiranica oil are hedycaryol CisH260 (17.37 %) and B-selinene CisH24 (8.71%). germacrene-D C13H24 (13.84%) and sabinene C10H16 (9.97%) were the major components of B. hirtum oil. P. chamaerapistrum oil was a mixture of (2S) and (2R) 2-methyl-3-butenenitrile C5HN, but the S isomer is the major
product. On alkaline hydrolysis of the oil, an amide (2-methylbut-3-enamide) and an acid
(2-methylbut-3-enoic acid) were obtained.
The oils of C. austroiranica and B. hirtum inhibited the growth of Staphylococcus
aureus, Escherichia coli and Candida albicans but inactive against Pseudomonas
aeruginosa. Combined oils of C. austroiranica and B. hirtum (5 uL + 5 uL) inhibited the
growth of S. aureus, E. coli and C. albicans but also lacked activity against P. aeruginosa.
The oil of P. chamaerapistrum was active against all the tested microorganisms at 1000
ug/ml in the agar - dilution - streak method and 142.9 ug/ml in the well - diffusion
method. These results supported the practice of using fresh plant materials to ward of skin
infection or suppress body odor in Oman folklore medicine.
