English abstract
A simple, fast, sensitive, versatile and environmentally friendly method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (COSCI) and HPLC separation on monolithic columns. Phenols react with (C6$CI) within 3.0 minutes in the microfluidic in ambient temperature to produce phenol-coumarin derivatives which were separated in RP-HPLC followed by post-column ring opening and fluorescence detection at hexc = 360 nm, and hem = 460 nm. The optimum conditions for the derivatization, separation, and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of (0.75 - 7.5 mg 1). The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds
