The coastline in Sultanate of Oman is rich in different raw natural materials such as seaweed plants. Red seaweeds contain various nutritional compositions such as carbohydrates, protein, minerals, vitamins, polyphenols, and lipids. These compounds are used in various applications in food industries or pharmaceutical, nutraceutical, or cosmetic applications. Hypnea bryoides species belonging to the Rhodophyta group (Red seaweed) were found to contain a good amount of protein. This study aimed to determine the proximate composition of the species Hypnea bryoides, extract its organic solvent soluble compounds and investigate its polyphenols contents (TPC, TFC, and TTC), and extract protein and study its functional and chemical properties. The non-water-soluble molecules were extracted using five different organic solvents sequentially (Hexane, DCM, ethyl acetate, acetone and methanol). The protein was extracted in an alkaline solution (0.3 M NaOH, pH 12). High-Performance Liquid Chromatography (HPLC) was performed to study the molecular weight of protein and amino acid composition and profiling. The structural characteristics of the organic solvent extracts and protein were analyzed by Fourier Transform Infrared (FTIR-ATR) technique. The finding of this study showed that the carbohydrates (67.57 ± 0.00%) were the most abundant components in H. bryoides, followed by protein (19.48 ± 0.07%), and the lipid content was found the lowest (1.01 ± 0.07%). The ash content was 11.87 ± 0.05%, and the main essential minerals found in the sample were Na, K, Ca, P, Fe, where the Na was detected with the highest concentration (30.19 ± 0.43 mg/g). The methanolic extract exhibited the highest polyphenols contents (141.3 ± 0.59, 115.11 ± 3.25 and 36.77 ± 1.05 mg/g of TPC, TFC and TTC, respectively) among the five fractions. The total yield of protein was 6.00 ± 0.35% and the purity was 88.50 ± 0.71%. The protein showed a higher oil holding capacity (13.56 ± 0.26 g oil/ g protein) than water capacity (9.61 ± 0.15g water/g protein). The best protein solubility, emulsifying capacity, emulsifying stability, and foaming capacity were observed at pH 8 and 10 (P≤0.05). However, pH 4 revealed the best foaming stability. The in-vitro digestibility of the protein extract was 62.62 ± 3.29%. In addition, the structural characteristics analyzed by FTIR-ATR demonstrated presence of α-helix (1,638.2 cm-1 ) and β-sheet (1,537.92 cm-1 ) structures in the protein extract. Different protein molecules with a wide range of MWs (0.4 -125.2 kDa) were detected by HPLC. The result of amino acid profiles showed that the essential amino acids counted for 35.08% of the total amino acids; and methionine + cysteine was the limiting amino acid (Amino acid score: 0.003). In conclusion, the organic solvent extracts from H. bryoides were found with high polyphenols content. The protein content of this seaweed showed great functional and chemical properties. Therefore, the isolated protein can be utilized in different food products as an emulsifying or foaming agents. Moreover, future work could be conducted to focus more on nutritional value (i.e. protein quality and digestibility) and biological activities of H. bryoides protein.