Seed oils contain fatty acids and minor components that are important to cosmetic and skin care products. Some seed oils obtained from plants which cultivated in Oman are used traditionally as skin lotion and for medical purposes. In this investigation, oils from Aeridocarpus orientalis, Citrullus colocynthis, Cucumis sativus, Moringa peregrina and Punica granatum were extracted from dry seeds and analyzed using a combination of 4,4-dimethyloxazoline derivatives and gas chromatography-electron impact mass spectrometry (GC-MS) methods. The most abundant unsaturated fatty Is detected in the seed oils were (C18:1) (9) from A. orientalis and M. peregrine, (C18:2) (9, 12) from C. colocynthis and C. sativus, and (C18:2) (11,14) from P. granarum, respectively, Saturated fatty acids with carbon atoms in the range C16 to C22 were also found in some of the oils. The structures of the fatty acids were deduced from fragmentation profile of their 4, 4-dimethyloxazoline derivatives. The oils were also evaluated for antioxidant properties using radical scavenging assays: 2,2-diphenyl-1-pierylhydrazyl (DPPH), 2',2'-azinobis (3-ethylbenzothiazoline-6 sulfonic acid) (ABTS) assay and B-carotene bleaching method. P. granarum and M. peregrina oils with unconjugated acetylenic fatty acids demonstrated the best antioxidant capacity and could protect the skin from oxidative damage when incorporated into skin care products.