Background: Integrons are semi-mobile elements that have a significant role in the dissemination of antibiotic-resistance-conferring genes between bacteria. This study aims to describe the prevalence and characterize the molecular features of integrons in clinical isolates of Klebsiella pneumoniae. Methods: A total of 50 multidrug-resistant representatives K. pneumoniae isolates from Sultan Qaboos University Hospital (SQUH) were investigated for the presence of integrons class 1, 2, and 3 by Polymerase Chain Reaction (PCR). Antimicrobial susceptibility test was performed by disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The variable regions of the integrons were PCR-amplified and sequenced to detect the gene cassettes. To assess the horizontal transferability for the gene cassettes, filter- mating conjugation experiments were conducted. Whole genome sequencing (WGS) was performed in representative (n=23) isolates and analyzed using various bioinformatics tools, to determine the sequence type (ST), acquired antimicrobial resistance genes, and perform plasmid-typing of integron-carrying strains. Highly virulent serotypes of K. pneumoniae were identified through amplifying 5 capsule serotype genes from the integron-positive isolates. Results: Class 1 integron was detected in 96% (48/50) of isolates, whereas, class 2 and class 3 integrons were not detected. Among integron-positive isolates, 18 stains contained variable regions. All the representative samples that were tested for the horizontal transferability, confirmed the successful transfer of integrons between cells and WGS confirmed their presence in conjugative plasmids. Ten different gene cassette arrays were detected with dihydrofolate reductase (dfrA14) as the most prevalent (34.8%) gene cassettes in class 1 integrons. Furthermore, MLST analysis detected 9 sequence types (ST), with ST-231 and ST-395 as the predominant STs. BlaOXA-232 and blaCTX-M-15 were the most frequently detected carbapenems and betalactamases in sequenced isolates. Three isolates were classified as highly virulent serotypes (k1 and k2). Conclusions: This study highlighted the widespread of class 1 integrons among MDR isolates of K. pneumoniae and confirmed the spread of antimicrobial resistance genes by horizontal transfer. Therefore, the wise use of antibiotics and the adherence to effective infection control measures are necessary to limit further dissemination of multidrug-resistant organisms.