High-reliability organizations (HROs) of healthcare are facing tough challenges to improve nurses’ psychological safety and intent to stay through maintaining organizational resources, work flexibility, environmental safety, and effectively managing stress and anxieties the nurses face in the workplace. Aim: This study aims to identify the relation between perception of working at HRO, psychological safety and intent to stay among Dar Al-Fouad Hospital nurses. Design: A correlational ex-post facto research. Setting: The study was conducted at Dar Al Fouad Hospital. Study subjects: A simple random sample of nurses who are working in Dar Al Fouad Hospital n=225. Tools: Three instruments were used in this study: the instrument one Part (1) - personnel data, Part (2) - Safety Organizing Scale; instrument two - team psychological safety scale, and instrument three - nurses’ intent to stay scale. Results: The total perception levels of HRO was moderate 64.5%, psychological safety was moderate 75.6%, and intent to stay was moderate 89.3% among the studied sample. Conclusion: There was negative statistical significance relation between staff nurses’ total perception level regarding HRO, and psychological safety and intent to stay. Recommendation: Health care managers at HRO work to improve nurses’ psychological safety and intent to stay through providing personal and professional support, encouraging to report mistakes, ask questions, receive effective feedback, and see mistakes as opportunities to learn.