Pharmaceutical and scientific communities have recently focusing on medicinal plants and various publications have documented the therapeutic effects of natural compounds to validate the claims of their biological activity. The plant natural compounds possessing effective antimicrobial agents since most of the pathogenic bacteria are becoming resistant to existing synthetic antibiotics. The miracle herb selected for this study is Nigella sativa, which has been examined for antimicrobial and antioxidant effects, phytochemical analysis and GC-MS characterization. Five pathogenic bacteria and two fungi were examined for antimicrobial effect. The Gram positive bacteria were Staphylococcus aureus and Bacillus subtilis, whereas the Gram negative bacteria were Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi. The selected fungi were Aspergillus flavus and Candida albicans. Five different solvent extracts have been prepared, which are aqueous, ethanol, chloroform, hexane and cyclohexane. The results showed that all the extracts, except aqueous, were able to inhibit the microbial growth with an inhibition zone as high as (20.6 ± 0.5 and 20.5 ± 0.5 mm) for E. coli and S. aureus respectively. In addition, all the extracts were showed antioxidant effects at varied concentrations. As a result of the phytochemical analysis and GCMS characterization, the extracts were contained a wide range of phytochemical constituents and bioactive compounds responsible for antimicrobial and antioxidant effects. The findings of the study revealed that the N. sativa plant has potential phytoconstituents which stimulate its biological activities, therefore this experimental plant can be further assayed for pharmacological preparations.