Document
Cytokine profile of sickle cell disease in Oman.
Identifier
DOI: 10.1002/ajh.20196
Source
American Journal of Hematology. V. 77, 4, P. 323-328
Contributors
Al-Kindi, Salam. , Author
Alnaqdy, Adel Alwan. , Author
Daar, Shahina., Author
Knox-Macaulay, Huxley., Author
Dennison, David., Author
Country
United States.
City
New Jersey
Publisher
Wiley-Liss, Inc.
Gregorian
2004-12-01
Language
English
Subject
English abstract
The aim of our study was to assess the cytokine profile of sickle cell disease (SCD) patients in steady state and in vaso-occlusive crisis (VOC). VOC has a complex nature, involving interactions between sickle red blood cells (RBC), the endothelium, and leucocytes. Endothelial damage due to recurrent adhesion of sickle RBCs may disrupt endothelial function, leading to altered cytokine release. It is therefore pertinent to study the cytokine profile of SCD patients in steady state and in crisis prior to exploring its contribution to vaso-occlusive manifestations, since it is believed that an altered balance of proinflammatory and anti-inflammatory cytokines plays an important role in painful crisis. Cytokines including IL-1β, IL-2, IL-4, IL-6, IL-8, TNF-α, and IFN-γ were measured by commercially available ELISA kits in SCD patients (n = 60); in steady state (n = 26) and in painful crisis (n = 34) and compared with nonanemic age- and sex-matched normal Omani controls (n = 20). SCD patients in crisis showed elevated levels of TNF-α (P 0.092) and IL-6 (P 0.024) when compared with steady state. It was also observed that SCD patients in steady state showed a significant elevation in IL-1β (P 0.04), IL-6 (P 0.0001), and IFN-α (P 0.02) as compared to normal subjects. It is thus evident that both type I and type II cytokines are significantly altered in SCD patients. In steady state, type II proinflammatory cytokines are elevated, whereas in crisis, an additional augmentation of type I cytokines occurs, with persistent elevation of type II cytokines, emphasizing the role of perturbed endothelium and activated monocytes in the pathophysiology of vaso-occlusion in sickle cell crisis.
Description
American Journal of Hematology
Volume 77, Issue 4, December 2004, Pages 323-328
Volume 77, Issue 4, December 2004, Pages 323-328
ISSN
0361-8609
Category
Journal articles