Document

First report of tomato leaf curl Albatinah virus (Tolcabv) and its associated betasatellite infecting papaya in Oman.

Identifier
DOI: 10.1094/PDIS-09-14-0912-PDN
Author
Contributors
Al-Shihi, A., Author
Amin, I., Author
Publisher
American Phytopathological Society.
Gregorian
2015-03
Language
English
English abstract
Leaf curl disease with severe curling, vein darkening, and vein thickening was observed on papaya plants in a field in Qurayat district of Oman during December 2013. Disease incidence ranged from 50 to 70%, particularly in young papaya plants. The presence of a large population of whiteflies and symptoms observed on papaya plants suggested that the causal agent could be begomoviruses (family Geminiviridae) and associated satellites. Four leaf samples with mild and severe leaf curling were collected from the field. Total nucleic acid extracted from symptomatic and healthy plants using the CTAB method were used as a template to amplify circular DNAs using Φ29 DNA polymerase, and products were digested with restriction enzymes to identify fragments of 2.6 to 2.8 kb typical of geminiviruses. BamHI yielded fragments of ~2.8 and 1.4 kb when the digested products were resolved by electrophoresis on a 1% agarose gel. These fragments were cloned and sequenced using a primer walking strategy in both directions.
Member of
ISSN
0191-2917
Category
Journal articles