Microfluidic device or a lab on a chip has been explored as a tool for the development of a highly sensitive, fast and economic method for analysis of tranexamic acid (TA) and amlodipine besylate (AM) in pharmaceutical formulation through peroxyoxalate chemiluminescence (PO-CL) reaction. This reaction involves the oxidation of an oxalic acid derivative by hydrogen peroxide in the presence of a suitable fluorophore such as fluorascamine (FA). Where FA was reacted with TAJAM and the derivatization product (FA-TA or FA-AM) was used in bis-(2,4,6-trichlorophenyl) oxalate (TCPO-CL) system. The parameters that affect the CL signal intensity were carefully optimized. These include pH, concentration of reagents used, the flow rates and instrumental setup. Also, the effect of imidazole, commonly used as a catalyst in PO-CL system was extensively investigated and the effect of different surfactants in CL signal intensity. A linear range calibration curve between (5-900 ng mL-?) and (10-100 ng ml") was achieved under optimum conditions for TA and AM respectively. The Limit of detection (LOD) was found to be 1 ng mL-1 for TA while for AM its equal to 3 ng mL-'. Also the limit of quantification (LOQ) was 3 ng ml.* and 10 ng mL-' for TA and AM respectively. This method was found to be fast as it takes less than 120 runs per hour to be carried out and economically good consuming only 0.3 ug of FA and 2.5 ug of TCPO per run. Finally, the method was practical for analysis of TA and AM in pharmaceutical product with a nearly 100% recovery CL signal intensity from other interfering ingredients normally present in these products.