In this project, different types of carbon-based quantum dots (C-based QDs) were synthesized by simple and green routes using safe precursors. They were utilized to obtain highly sensitive and powerful multi-emission chemiluminescence (CL) systems. Several attempts have been made to reach this goal through the development of three systems: bis(2, 4, 6-trichlorophenyl) oxalate (TCPO)-hydrogen peroxide (H₂O₂) CL system, sulfite (𝑆𝑂3 2−)-acidic permanganate (KMnO4) CL system and polyphosphate (PP)- acidic KMnO4 CL system, each in the presence of a certain type of C-based QDs. These CL reactions were carried out on a small square shaped chromatography paper substrate, prepared by a paper cutting fabrication technique and 3D-printing, ending with the development of a paper-based analytical device coupled with CL detection technique (PAD-CL). Out of the three CL systems, two showed multi-emission peaks with well separated wavelengths. Careful optimization of the instrumental setup and various experimental conditions were exploited in order to obtain the strongest emission and highest sensitivity. Several experimental parameters were thoroughly optimized, including the size of detection paper, the order of injecting the analyte and CL reagents, the concentrations and volumes of various reagents and C-based QDs. The CL behavior of C-based QDs were investigated and the CL mechanism of each developed system was studied to clarify the role of QDs in the development and enhancement of the multi emission CL systems. The analytical application of the developed systems is an important part to complete the picture of the work. In this regards, the established CL systems were tested for different analytical applications. Under optimal experimental conditions, good linear relationships were observed between the studied analyte concentration and the CL intensity of the established CL system. The designed assays are characterized by their low-cost, ease of use, facile but accurate, and high selectivity for the detection of different analytes in various spiked food and environmental samples. Besides, the portability of these probes makes them suitable for on-site screening purposes.