Constructed wetlands have been proven effective in treating the significant amounts of produced water generated from oil extraction operations. However, with the increasing use of partially hydrolyzed polyacrylamide (HPAM) in enhancing oil recovery, and its natural degradation into its toxic acrylamide (AM) monomer, the negative impacts of AM on the biological processes of wetlands have been gaining increased research attention. Microbial mats in wetlands play a key role in the bioremediation of hydrocarbons present in produced water, and the effect of this AM contamination on the mats is still understudied. This project aims to investigate the impact of AM on microbial mats, and their potential for AM degradation under aerobic and anaerobic conditions at different AM concentrations, using GC and HPLC techniques, respectively. Additionally, AM degradation by pure bacterial and algal strains was explored. Results showed that AM generated CO2 in the mat treatment groups (500, 1000, and 2000 mg/L AM), indicating that AM was being used as a carbon source. The mats were also found to degrade AM efficiently within 28 days of incubation under aerobic conditions, while very slow rates were observed under anaerobic conditions. Aerobic mat incubations at 500 mg/L and 1000 mg/L AM resulted in 95 ± 0.1% and 90 ± 10.6% AM degradation, respectively. Moreover, independent growth of bacterial and algal isolates in AM revealed interesting degradation efficiencies, where some strains have fully degraded AM up to 1000 mg/L after 7 days and 34 days, respectively. It was concluded that wetland microbial mats adapt well to AM and could maintain their respiration and hydrocarbon degradation activities in the field