English abstract
Objective and Method – To investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3•–
end of a primer to amplify a 268 bp (base pair) region of the human •–globin gene using different annealing temperatures
(45 to 65•C). Results – The primer with the G/T mismatch was as efficient as the normal primer (G/C match) in the
amplification of a 268 bp product at all temperatures tested. However, the primers having G/A or G/G mismatches at the 3'-
end did not produce any specific polymerase chain reaction (PCR) fragment at all the annealing temperatures used, except a
barely detectable 268 bp product for the G/G mismatch at 45 and 50•C. Conclusion – We conclude that our PCR system was
refractory to amplification when one of the primers contained a G/A or G/G mismatch at the 3•–end with template DNA.
