This study aims to identify the strategic leadership practices (setting strategic direction, developing human capital, participatory decision-making, supporting organizational culture) and their application in the Ministry of Trade, Industry and Investment Promotion. It also aims to measure employees' motivation levels and examine the degree of achieving institutional excellence. Specifically, it seeks to measure the impact of strategic leadership practices and employee motivation on organizational excellence. The researcher employed the descriptive analytical approach to conduct the study and the questionnaire as the primary tool for data collection. The ministry's employees represented the study population, and the convenient sampling method was used to collect data from a sample of 262 employees. The researcher also used the SPSS program for statistical analysis of data. The study's findings revealed a positive and significant impact of strategic leadership practices on institutional excellence and that setting strategic direction was the most influential dimension of strategic leadership, followed by supporting organizational culture, human capital development, and participatory decision-making. Moreover, the results indicated the presence of a positive and significant impact of employee motivation on institutional excellence.