The present study was conducted using geometric morphometric analysis of otolith to discriminate stocks of the predominant species of genus Acanthopagrus in the west and east of Strait of Hormuz. Samples were taken from 5 regions in the Persian Gulf and Oman Sea from March 2016 to March 2018. The 145-otolith samples were obtained from the Arabian yellowfin sea bream (Acanthopagrus arabicus). The results of this investigation confirmed the existence of two separate populations in the west and east of the Strait of Hormuz.