A field experiment was carried out at Al- Batinah International Company (BIC), Al- Suwiaq, Al-Batinah Region, Sultanate of Oman during 2005-2006. The objective of the study was to study the effect of different rates of a combination of N, P and K fertilizer, desuckering to one or two suckers per mat and their interaction on growth and yield of 'Williams' banana (Musa accuminataColla). A split plot design was used with one or two suckers per mat as main plot and a combination of three levels of chemical fertilizers, i.e. N (300, 600 and 900 g/ mat/yr.), P (50, 100 and150 g/mat/yr.) and K (250, 500 and 750 g/mat/yr.) in addition to non-fertilized control (no NPK applied) treatment as sub-plots treatments. The fertilizer treatments were arranged in a Completely Randomized Blocks Design with three replication mats. Different combinations of chemical fertilizer resulted in significant difference in both vegetative growth and yield of banana. The vegetative growth of non-fertilized plants was significantly higher in all measured parameters compared to fertilized banana plants. Fertilizing with NPK at the levels of 600, 100 and 500 gm/ mat / yr., respectively, had significantly better yield and post harvest characteristics under either one or two suckers per mat than all other treatments. For this fertilizer treatment, the total bunch weight, middle hands weight and number of fingers per middle hands were 14.98 kg, 13.47 kg, 1.365 g and 16.05 respectively. Despite the numerically higher fruit production with two suckers per mat compared to one sucker, there were no significant differences in growth, yield or fruit quality. There were little differences among other fertilizer treatments on the vegetative and reproductive growth, yield and fruit quality of 'Wiliams' Banana. From the results of the present study, the recommended rate of NPK fertilizer for 'Wiliams' banana in Al-Batinah region of Oman is 600, 100 and 500 g/mat/yr.